Method for the estimation of Morindin content

Method for the estimation of Morindin content

Reagent Required:

Methanol L.R. Grade, Sulphuric acid, Ammonia solution.

Glass wares and Apparatus Required:

250mL Conical flask, Gooch crucible, 50mL Stoppered cylinder and Evaporating dish.

Procedure:

Weigh accurately about 5.0gm of dry extract powder into a 250mL RB flask, Add about 25mL of  90% methanol . Reflux for 30minutes. Cool & filtered through whatman No.1.Repeat the same two times more. Remove solvent by distillation up to soft mass. Cool &  reflux successively with 25mL each of Petroleum ether, chloroform, carbon tetrachloride & ethyl acetate. Cool  & discard the organic layer by decantation. Dissolve the residual extract in 25mL of methyl alcohol. Filter through whatman filter paper & concentrate up to 5mL.. Filtrate is slowly added with constant stirring to 25mL of acetone into a preweighed dish. Left overnight to get precipitate. Decant the supernatant liquid & discard. Dry the precipitate at 105°C to constant weight & weigh.  

Calculation:

Percentage of Morindin content is calculated as:

              (A – B) X 100 

        =  ----------------------

                       W    

A   = Weight of residue and dish

B   = Weight of empty dish

W  = Weight of material taken