Gymnemic acid content

Method for the estimation of  Gymnemic acid content

Reagent Required:

2N Sulphuric Acid, N/10 NaOH solution

2N Sulphuric Acid :-13.5mL of Sulphuric acid (LR) in water and make up to 250mL.

N/10 NaOH Solution: Dissolve 2gm sodium Hydroxide pellets in 500mL distilled water.

Glass wares and Apparatus Required:

 Beaker 250mL, Glass road, Filter paper Whatman No.1

Procedure:

Weigh accurately about 1.0gm of dry extract powder in a beaker and add 50mL of water and stir. If the material is not completely dissolve add 10mL of N/10 NaOH and shake well. Filter through ordinary filter paper. Wash filter paper with 10mL of water and add it to the above filtrate and add carefully 2N H₂SO₄ with constant stirring upto pH 2.0. Allow the precipitate to settle for 1 hour. Filter under vacuum and collect the precipitate on a previously weighed whatman No.1 filter paper. Dry the precipitate in an oven  to constant weight, cool in desiccators and weigh.

   

Calculation:

Percentage of Gymnemic Acid Content is calculated as:

             (A – B) X 100 

          =----------------------

                     W

A = Weight of filter paper with Gymnemic acid

B = Weight of filter paper

W = Weight of material taken

Note : For 25% of Gymnemic acid sample should be taken 3.0 gm.