Andrographolides content

Method for the estimation of  Andrographolides content

Reagent Required:

Methanol, Benzene, Ethyl acetate (LR Grade)

Glass wares and Apparatus Required:

R.B. flask, condenser, soxhlet apparatus, funnel, filter paper No. 1, glass rod, Evaporating dish.

Procedure:

Weigh accurately about 3.0gm of dry extract powder in a small soxhlet apparatus with 200mL of methanol. Evaporate the extract to 50mL on a water bath and add 30mL of water. Wash the aqueous alcoholic extract with two 25mL portions of benzene and to the aqueous alcoholic extract add 20mL of water and extract with four 25mL portions of ethyl acetate. Combine the ethyl acetate extracts dry to constant weight at  90⁰ C and weigh the andrographolide.

Calculation:

Percentage of andrographolide content (w/w) is calculated as :

(A – B)  X 100

          W

Where:

A   = Weight of andrographolide + empty dish

B   = Weight of empty dish

W  = Weight of sample taken

For raw material: 10gm sample (20 #) is to be taken in the case of raw material and the quantity of methanol shall be increased accordingly. Rest of the method is same as above.