Curcuminoides content (By UV)

Method for the estimation of Curcuminoides content 

Reagent Required:

Methanol LR Grade

Glass wares, Apparatus & Instruments required:

Volumetric Flasks -        - 100mL, 50mL

Beakers -                      - 100mL, 50mL

Spectrophotometer.

Procedure:

Take accurately about 60mg of the Curcumin powder in a 100 mL volumetric flask. Dissolve it completely in methanol and make up the volume up to the mark (Solution A). Take 5mL solution A into the 50 mL volumetric flask and make up the volume with methanol (Solution B). Take 2mL solution B into the 50 mL volumetric flask and make up the volume with methanol. Shake well and take absorbance on 425nm. Calculate the %age of curcumin content taking E1% as 1680 & using methanol as blank.

Percentage of curcuminoides content may be calculated as:-

                                            Abs x 100 x 50 x 50

% of Curcuminoides =  ------------------------------------------

                                           1680 x weight taken x 5 x 2

For Raw material:-  

Weigh accurately about 5gm of sample powder (20#) into a 250mL R.B. flask. Add 50 mL of methanol L.R. Grade and reflux on water bath for 30minutes.  Cool & filter into a 100mL volumetric flask. Repeat the process in the same manner. Make up the volume to 100mL with the same solvent. Further dilute so as the absorbance should be around 0.420 to 0.450. Rest the procedure is same as above.