I PURPOSE
To describe the procedure for
operation of anerobic jar
II SCOPE
This
procedure is applicable to Anaerobic jar in Microbiology Department….
III. RESPONSIBILITY
Microbiologist – Quality Control
IV. ACCOUNTABILITY
Incharge - Quality Control
V.
PROCEDURE
1.0
Anaerobic system consists of a jar (polycarbonate), an
outer lid with thumbscrew, an inner lid with o-ring gasket, three catalyst
reaction chambers, catalyst replacement charges/gas pack plus envelopes, one wire
rack, and test tube rack.
2.0 Using of catalyst replacement charges
2.1 Principle: Oxygen is
removed from the jar by catalytic reaction. The catalyst activates Hydrogen gas, introduced
into jars by activation of Gas Pack disposable Hydrogen
+carbon dioxide generator envelopes to combine with free oxygen in the jar to form water, which may be
observed as condensate on the inner wall
of the jar.
2.2 Arrange the required plates / tubes in the
anaerobic jar.
2.3 Unscrew the catalyst chamber from
underside of gas pack lid and remove catalyst.
2.4 Place one bag of fresh catalyst pellets
(2.5 ± 0.5 g) in the chamber.
2.5 Hold the lid underside down, and screw the
chamber in to the threaded hole in the
lid. Do not invert chamber while performing this operation. If the chamber is inverted. Pellets may fall in to the threaded
hole and be crushed. This could produce
pellet fragments and dust
which may subsequently escape through the screen
and into the jar when the jar is being operated. Avoid this possibility by maintaining
the chamber in a “thread up-screen down” position when tightening it into place
on the bottom of the lid.
2.6 As a further safety precaution before each
use. Gently shake the lid to assure that any
dust, which may be present, will fall from the screen prior to placing the lid on jar.
3.0 Using of gas pack plus envelopes
3.1 Principle: The gas pack plus envelope is
activated by the addition of water, which passes
through a series of channels to a
filter paper wick. The wicks feeds the water
to the gas- generating tablets in the
tablet chamber. Hydrogen generated from
a sodium borohydride tablet
following the addition of water,
combines with the oxygen in the jar in the of the palladium catalyst to form water.
3.2 If using the gas pack plus envelopes there
is no need of using catalyst replacement
charges or viceversa.
3.3 Arrange the required plates / tubes in the
anaerobic jar.
3.4 Peel open the corner of the envelope along
the dotted line and place the envelope in
the clip of the rack with the catalyst
sachet toward the outside. Three envelopes
should be used for one run of the
anaerobic system.
3.5 Add 10 ml of tap, distilled or de-ionized
water through the open / cut corner of the
envelope. Use a pipette or syringe
inserting only the tip in to the open corner. Never
push object down in to the envelope.
3.6 Close the gas pack jar promptly after
envelope is activated and finger tighten the
lid clamp in the manner described in the insert directions for the system
being used. Incubate at conditions appropriate for the organisms being
cultured, but not above 42°C.
3.7 After use, open the jar and allow it to
aerate for approximately 15 seconds prior to
removing the rack with
its contents. Do not handle the gas Pack envelope until after the catalyst, the
use of palladium catalyst pellets in the catalyst reaction chambers in the lids of Gas Pack jars is not required).
4.0
Each gas pack system should be tested periodically for
its ability to provide adequate conditions for the growth of anaerobic bacteria.
5.0
If visible condensation does not occur with in 60 min.
after the gas pack system is started then the reaction should be terminated by
opening the jar. Check the condition of the
lid and o-ring gasket for the leakages.
6.0
The
system must contain fresh or rejuvenated catalyst if envelopes with out
integral catalyst are used and the system should
be maintained upright all times.
VI. REVISION
SUMMARY
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Version No.
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Revised on |
Reason for Revision |
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01
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NA
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NA |
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