Method for the estimation of Total Gingerols content
Reagent Required:
Methanol
LR Grade
Glass wares,
Apparatus & Instruments required:
Volumetric
Flasks 100mL, 50mL, 25mL
Beakers
50mL,
Pipettes
– 5mL
U-V
Spectrophotometer
STANDARD PREPARATION:
Weigh
accurately about 100mg of working standard into a 100mL volumetric flask. Add
about 25mL of methanol. Ultrasonicate till dissolved and make up the volume
with methanol. Further dilute 5mL to 25mL with methanol (Solution B).
SAMPLE PREPARATION:
Weigh
accurately about 100mg of sample into a 100mL volumetric flask. Add about 25mL
of methanol. Ultrasonicate till dissolved and make up the volume with methanol,
warming if necessary. Make up the volume with methanol. Further dilute above 5mL
solution to 25mL with methanol. Ensure that the active concentration in the
sample is approximately similar to that of standard concentration. Shake well and filter through what man No.1
filter paper.
.
PROCEDURE:
Determine
absorbance of both standard and sample solution at wavelength of 280nm.
Percentage
of Total Gingerols content is calculated as:
Absorbance of sample X Concentration of std
X purity of std
% of Gingerols =----------------------------------------------------------------------------------------------
Absorbance of standard X
Concentration of sample
For Raw Material: -
Weigh accurately about 10.0gm Raw herb of Ginger in
250 mL R.B.Flask. Add about 75mL of methanol and reflux for one hour. Cool and
filter the solution. Rinse the residue in the flask with small quantity of
methanol & filter through same filter paper and make up the volume with
methanol. Further dilute above 5mL solution to 50mL with methanol. Further
dilute above 5mL solution to 100mL with methanol. Ensure that the active
concentration in the sample is approximately similar to that of standard
concentration. Shake well and filter
through what man No.1 filter paper.
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