I PURPOSE
To describe the procedure for the
operation of Autoclave
II SCOPE
This
procedure is applicable to Autoclave in
Microbiology Department…...
III. RESPONSIBILITY
Assistant - Microbiologist .
IV. ACCOUNTABILITY
Incharge - Quality Control
V.
PROCEDURE
1.1 Ensure that the equipment is clean.
1.2 Connect the equipment to the main power.
1.3 Ensure that the equipment is properly
validated and has validation tag.
1.4 Fill DM water upto the required level as
per marking.
1.5 Load the material according to the
validated loading pattern as per VP102.
1.6 Close the door properly.
1.7 Test tubes, flasks are plugged with
non-absorbent cotton and covered with butter paper to
avoid the drenching of the plugs during the release of steam when condensation occurs.
1.8 Switch “ON” the “mains”.
1.9 Switch on the autoclave.’
1.10
Main status indicators- red lamp (indicates equipment
is on) and green lamp (Heating coils
cut off) glow.
1.11
Open the steam valve of the chamber when the jacket
pressure is 1.2 Kg/cm2. which is displayed
on pressure gauge (Jacket).
1.12
Open the Exhaust until all the air is expelled out
(This is to avoid the formation of air pockets
in the chamber) and close the exhaust.
1.12.1
Repeat the step 5.12 as per the validated cycle to
achieve 121°C and 15 lbs, which are displayed on
temperature gauge and pressure gauge (chamber) respectively.
1.13
After attaining the 121°C and 15 lbs., run the cycle
for Sterilisation for a validated period depending
up on the loading pattern.
1.14
After Sterilisation cycle close the steam valve of
chamber.
1.15
Then open the vacuum exhaust valve.
1.16
When inside pressure is Zero (Which is displayed on
pressure gauge) then open the aseptic
side door as per the requirement and unload the materials.
1.17
Record the cycle details in the Autoclave register
VI REASON
FOR REVIEW
Periodic
review
4.0 List of Formats
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Format No.
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Title |
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VI. REVISION
SUMMARY
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Version No.
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Revised on |
Reason for Revision |
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00
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NA
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NA |
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